mouse anti human pcna  (Boster Bio)

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Antibodies used for Western blots.

Article Snippet: Mouse anti-human PCNA , Wuhan Boster Bio, China , BM0104 , 1:1000.

Techniques: Western Blot

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Effects of BFA and TM on the expression of PCNA in HepG2 cells. HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The protein levels of PCNA were determined using Western blot assay. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05 vs. control group; # P < 0.05 vs. DMSO (0.1% v/v, vehicle control) group. Abbreviations: BFA, brefeldin A; DMSO, dimethyl sulfoxide; PCNA, proliferating cell nuclear antigen; SD, standard deviation; TM, tunicamycin.

Article Snippet: Mouse anti-human PCNA , Wuhan Boster Bio, China , BM0104 , 1:1000.

Techniques: Expressing, Western Blot, Control, Standard Deviation

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Antibodies used for Western blots.

Article Snippet: Mouse anti-human PCNA , Wuhan Boster Bio, China , BM0104 , 1:1000.

Techniques: Western Blot

Journal: Journal of Medicine and Life

Article Title: Caffeic acid phenethyl ester attenuates Enterococcus faecalis infection in vivo: antioxidants and NF-κB have a protective role against stomach damage

doi: 10.25122/jml-2023-0544

Figure Lengend Snippet: Immunohistochemical staining of PCNA among mice in different study groups, in the non-glandular forestomach (upper row) and glandular stomach (lower row). The control group shows minimal to no PCNA immune reactivity in the non-glandular and glandular parts. Group II, infected with Enterococcus faecalis at a dose of 10 6 CFU shows increased PCNA immune reactivity in both stomach parts. Group III, treated with CAPE, shows a marked reduction in PCNA immune reactivity in the non-glandular and glandular parts of the stomach. Original magnification 100×.

Article Snippet: For antigen retrieval, the sections were immersed in tris-buffered saline (TBS) with a pH of 6 and heated in a microwave oven at 750 W. After cooling to room temperature, the sections were treated with MCM7 monoclonal mouse anti-human primary antibodies (Thermo Fisher Scientific) and PCNA monoclonal mouse anti-human antibodies (Thermo Fisher Scientific) at a dilution of 1:2.000 for 1 h. Then, the sections were treated with Dako Envision following washing in TBS.

Techniques: Immunohistochemical staining, Staining, Control, Infection

Journal: Journal of Medicine and Life

Article Title: Caffeic acid phenethyl ester attenuates Enterococcus faecalis infection in vivo: antioxidants and NF-κB have a protective role against stomach damage

doi: 10.25122/jml-2023-0544

Figure Lengend Snippet: Comparison of NF-κB and PCNA immunohistochemical staining among mice in different groups. A, NF-κB IHC staining area percentage. B, PCNA IHC staining area percentage.

Article Snippet: For antigen retrieval, the sections were immersed in tris-buffered saline (TBS) with a pH of 6 and heated in a microwave oven at 750 W. After cooling to room temperature, the sections were treated with MCM7 monoclonal mouse anti-human primary antibodies (Thermo Fisher Scientific) and PCNA monoclonal mouse anti-human antibodies (Thermo Fisher Scientific) at a dilution of 1:2.000 for 1 h. Then, the sections were treated with Dako Envision following washing in TBS.

Techniques: Comparison, Immunohistochemical staining, Staining, Immunohistochemistry

Journal: Journal of Medicine and Life

Article Title: Caffeic acid phenethyl ester attenuates Enterococcus faecalis infection in vivo: antioxidants and NF-κB have a protective role against stomach damage

doi: 10.25122/jml-2023-0544

Figure Lengend Snippet: Comparison of PCNA immunohistochemical staining among mice in different groups. Data expressed as percentage of PCNA-positive area.

Article Snippet: For antigen retrieval, the sections were immersed in tris-buffered saline (TBS) with a pH of 6 and heated in a microwave oven at 750 W. After cooling to room temperature, the sections were treated with MCM7 monoclonal mouse anti-human primary antibodies (Thermo Fisher Scientific) and PCNA monoclonal mouse anti-human antibodies (Thermo Fisher Scientific) at a dilution of 1:2.000 for 1 h. Then, the sections were treated with Dako Envision following washing in TBS.

Techniques: Comparison, Immunohistochemical staining, Staining

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis .

doi: 10.1080/21691401.2024.2347377

Figure Lengend Snippet: Figure 2. The effect of anti-Ts7TMR scFv on A549 lung cancer and its preliminary mechanism. A. The effect of anti-Ts7TMR scFv on the proliferation of A549 cells in vitro. A549 cells were co-incubated with different doses of anti-Ts7TMR scFv (5, 25, and 45 μg) for 48h to detect cell proliferation by CCK-8 assay. B. The effect of anti-Ts7TMR scFv on A549 lung cancer growth in nude mice. Nude mice were injected subcutaneously with A549 cells and then treated subcutaneously with PBS, negative serum (Ns), anti-T. spiralis serum (Ts), and different doses of anti-Ts7TMR scFv (25, 50, and 100 μg) around the tumour for 10 consecutive days. C. Scoring of PCNA, BCL-2, and VEGF expression in tumour tissues by immunohistochemistry (400 ×). Expression of PCNA, BCL-2, and VEGF in tumours treated with PBS, negative serum (Ns), anti-T. spiralis serum (Ts), and different doses of anti-Ts7TMR scFv (25, 50, and 100 μg). Scale bar = 20 μm. *p < 0.05, **p < 0.01.

Article Snippet: Then mouse anti-human PCNA monoclonal antibody (60097-1-Ig, Proteintech), mouse anti-human BCL-2 monoclonal antibody (60178-1-Ig, Proteintech), and mouse anti-human VEGF monoclonal antibody (66828-1-Ig, Proteintech) were added separately dropwise and incubated at 37 °C for 30 min. Then the sections were incubated with biotin-conjugated Goat anti-mouse IgG at 37 °C for 30 min. Next, Streptavidin Immunohistochemistry kits (KIT-9710, maxim biotechnologies) and diaminobenzidine (dAB-0031, maxim biotechnologies) were added dropwise in sequence.

Techniques: In Vitro, Incubation, CCK-8 Assay, Injection, Expressing, Immunohistochemistry

Journal: Gels

Article Title: A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer

doi: 10.3390/gels10040252

Figure Lengend Snippet: Analysis of cell proliferation in neural invasion models. ( A ) Immunohistochemical detection for PCNA at different time points (2 and 7 days) in each experimental group. CTR: control group model, Pre-Inv: pre-invasive model, Inv: invasive model. Scale bar of upper and bottom magnifications = 200 and 50 µm, respectively. ( B ) Quantitative analysis results of the proliferative rates expressed as the percentage of positively stained cells. Error bars represent standard deviations.

Article Snippet: Mouse anti-human PCNA (Clone PC10) , Ready to use , EDTA, pH = 8, 95 °C for 25 min , Master Diagnostica, Granada, Spain (ref. MAD-000903QD).

Techniques: Immunohistochemical staining, Control, Staining

Journal: Gels

Article Title: A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer

doi: 10.3390/gels10040252

Figure Lengend Snippet: Antibodies and reagents used for immunohistochemical analyses.

Article Snippet: Mouse anti-human PCNA (Clone PC10) , Ready to use , EDTA, pH = 8, 95 °C for 25 min , Master Diagnostica, Granada, Spain (ref. MAD-000903QD).

Techniques: Immunohistochemical staining, Plasmid Preparation

Journal: Vaccines

Article Title: Humoral and Cellular Immune Responses Induced by Bivalent DNA Vaccines Expressing Fusion Capsid Proteins of Porcine Circovirus Genotypes 2a and 2b

doi: 10.3390/vaccines12030324

Figure Lengend Snippet: Expression of the immunogen by 4 different constructs. HEK-293A cells were transfected with 1 µg of the pVAX1 plasmids harboring 4 different immunogen constructs. Cells and medium were collected and tested for protein expression at 48 h post-transfection. ( A ) Schematic diagram of the protein products produced by the constructs bearing the fusion gene GI . Proteins PCV2b-2a and GI are cleaved during translation, which is mediated by F2A. Red arrow indicate furin cleavage site; blue arrow indicates F2A cleavage site. ( B ) Immunofluorescence assay detecting the PCV2b-2a immunogen expressed by the two constructs: PCV2b-2a and SS-PCV2b-2a. ( C ) Immunofluorescence assay detecting the immunogen and fusion cytokine GI expressed by the two constructs: PCV2b-2a-GI and SS-PCV2b-2a-GI. ( D ) Western blot analysis of the PCV2b-2a fusion protein expressed by all 4 constructs in the cell lysate. PCNA was also detected and used as an internal control. ( E ) Western blot analyses of the fusion cytokine GI expressed from the two constructs bearing the fusion gene GI . Both cell lysate and medium were tested. The PCV2b-2a, GI and PCNA proteins were detected using anti-V5 mAb, anti-IL-4 mAb, and anti-PCNA mAb, respectively, in both immunofluorescence staining and Western blot. Cell nuclei were stained with DAPI (blue fluorescence). In Western blot, the cells transfected with the plasmid pVAX1 expressing PCV2b-2a, PCV2b-2a-GI, SS-PCV2b-2a, and SS-PCV2b-2a-GI are indicated as 1, 2, 3, and 4, respectively. The HEK-293A cells transfected with parental plasmid pVAX1 were used as a negative control (-ve).

Article Snippet: In parallel, an internal control staining was also performed by using a 1:2000 diluted mouse anti-human PCNA (eBioscience, San Diego, CA, USA) as a primary antibody and a 1:10,000 diluted HRP-conjugated goat anti-mouse IgG antibody (Abcam) as a secondary antibody.

Techniques: Expressing, Construct, Transfection, Produced, Immunofluorescence, Western Blot, Control, Staining, Fluorescence, Plasmid Preparation, Negative Control